Streeten B W, Swann D A, Licari P A, Robinson M R, Gibson S A, Marsh N J, Vergnes J P, Freeman I L
Invest Ophthalmol Vis Sci. 1983 Jan;24(1):119-23.
Bovine and human zonules were found to be composed of noncollagenous acidic glycoprotein with a high cysteine content, double that previously reported. In reduced zonular fractions the most prominent peptide had a molecular weight (MW) of approximately 70,000. Lesser quantities of 170,000, 50,000, and 35,000 dalton peptides were also present and a variable number of lower MW bands, depending upon the degree of reduction and denaturation. A fraction of bovine zonules soluble in low ionic strength buffers contained primarily a peptide of approximately 50,000 daltons, often present as a doublet. Amino acid and hexosamine content of these two fractions was consistent with the presence of at least two different glycoconjugates, one a proteoglycan. Carbohydrate analysis of whole zonules suggested that these glycoconjugates include a sialofucose-containing glycoprotein and a lesser quantity of xylose-containing proteoglycan. The amino acid profile and peptide content of the zonules resembled that of elastic tissue microfibrils, increasing further the possibility of a close relationship between these two fibrils.
研究发现，牛和人的悬韧带由富含半胱氨酸的非胶原酸性糖蛋白组成，其含量是先前报道的两倍。在还原的悬韧带组分中，最显著的肽分子量（MW）约为70,000。还存在少量170,000、50,000和35,000道尔顿的肽，以及数量不等的低分子量条带，这取决于还原和变性的程度。一部分可溶于低离子强度缓冲液的牛悬韧带主要含有一种约50,000道尔顿的肽，常以双峰形式存在。这两个组分的氨基酸和己糖胺含量与至少两种不同糖缀合物的存在一致，其中一种是蛋白聚糖。对整个悬韧带的碳水化合物分析表明，这些糖缀合物包括一种含唾液酸岩藻糖的糖蛋白和少量含木糖的蛋白聚糖。悬韧带的氨基酸谱和肽含量与弹性组织微原纤维相似，这进一步增加了这两种纤维之间密切关系的可能性。
