Rhodes R H
Histochemistry. 1983;78(3):349-60. doi: 10.1007/BF00496622.
Eyes from adult rodents, rabbits and humans were fixed in formalin, embedded in paraffin and incubated with a horseradish peroxidase-conjugated lectin from Ulex europaeus to localize vitreous-body and zonular glycoconjugates. Rodent eyes had reaction product for peroxidase activity in fibrous structures in the posterior chamber, vitreous base and vitreous cortex. The zonules and the internal limiting membrane region of the retina also were stained. Rabbit eyes had more stained fibrous material in the vitreous base than rodent eyes and the attachment region of the zonules on the lens capsule, the anterior hyaloid membrane and tracts in the vitreous cortex were more heavily stained in rabbits. There was heavy staining of the thick vitreous base in the human eyes as well as staining of zonules, anterior hyaloid membrane and vitreous cortex. The localization of this lectin may be specific for fucose in glycoproteins or other glycoconjugates, although this was not demonstrated here. However, the location of lectin binding sites correlates well with known sites of uptake of tritiated fucose and tritiated glucosamine in rabbit eyes. Eyes from the larger species studied had more lectin-binding glycoconjugates in fibrous structures in the vitreous body than those from smaller species. The amount of glycoconjugate identified in some of the lectin-binding sites may be related to some extent to the degree of stress incident upon those sites.
将成年啮齿动物、兔子和人类的眼睛用福尔马林固定，石蜡包埋，并用来自欧洲荆豆的辣根过氧化物酶偶联凝集素孵育，以定位玻璃体和悬韧带糖缀合物。啮齿动物的眼睛在房水后腔、玻璃体基部和玻璃体皮质的纤维结构中出现过氧化物酶活性的反应产物。悬韧带和视网膜内界膜区域也被染色。兔子眼睛玻璃体基部的纤维物质染色比啮齿动物眼睛更多，并且兔子的悬韧带在晶状体囊膜上的附着区域、前玻璃体膜和玻璃体皮质中的条索染色更重。人类眼睛的厚玻璃体基部有大量染色，悬韧带、前玻璃体膜和玻璃体皮质也有染色。这种凝集素的定位可能对糖蛋白或其他糖缀合物中的岩藻糖具有特异性，尽管此处未得到证实。然而，凝集素结合位点的位置与兔子眼睛中氚标记岩藻糖和氚标记葡糖胺的已知摄取位点密切相关。所研究的较大物种的眼睛玻璃体纤维结构中的凝集素结合糖缀合物比小物种的更多。在一些凝集素结合位点鉴定出的糖缀合物数量在一定程度上可能与施加于这些位点的应激程度有关。
