Hsu S M, Ree H J
J Histochem Cytochem. 1983 Apr;31(4):538-46. doi: 10.1177/31.4.6827084.
Using the avidin-biotin-labeled peroxidase complex (ABC) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. Varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with Concanavalin ensiformis (Con A), Lens culinaris (LCA), Phaseolus vulgaris (PHA), Pisum sativum (PSA), Ricinus communis (RCA), and Triticum vulgaris (WGA) agglutinins, but no evidence of binding was observed with Dolichos biflorus (DBA), Bandieraea simplicifolia (BSA), Arachis Hypogaea (PNA), Glycine soja (SBA), Sophora japonica (SJA), and Ulex europaeus (UEA) agglutinins. Three major patterns of binding were seen: the reaction products occurred along the plasma membranes (membranous), were confined to one pole of the cell membrane (cap-like), or were present diffusely in cytoplasm (cytoplasmic). The cells showing membranous and cap-like staining patterns corresponded to the lymphoid cells, as did the cytoplasmic to plasma cell and macrophage-histiocytes. Cap-like staining was observed on the lymphocytes at B and T cell areas with all six lectins. Thus, the presence of cap-like staining may not be useful for discrimination between B and T cells. Membranous staining, in contrast, was limited to lymphocytes of follicles (B cells) with PSA and LCA, and to germinal center cells with PHA, WGA, Con A, and RCA also reacted with the membrane of T-cell. The cytoplasmic staining reaction of macrophage-histiocytes varied markedly from one lectin to the other. Our study indicates that the carbohydrate moiety of the cells retains their binding sites for lectins through routine processing, providing a means of valid retrospective studies. Furthermore, these observations suggest that each lectin, despite its identical inhibitory sugar, should be tested for its unique reaction pattern, which is not predictable from the data derived from cell suspension studies.
采用抗生物素蛋白-生物素标记的过氧化物酶复合物（ABC）法，研究了一组12种生物素标记凝集素在福尔马林固定、石蜡包埋的反应性淋巴结和扁桃体中的染色反应。在淋巴细胞以及巨噬细胞-组织细胞中，刀豆球蛋白（Con A）、小扁豆凝集素（LCA）、菜豆凝集素（PHA）、豌豆凝集素（PSA）、蓖麻凝集素（RCA）和小麦胚凝集素（WGA）凝集素呈现出不同程度的凝集素结合，但双花扁豆凝集素（DBA）、单叶豆凝集素（BSA）、花生凝集素（PNA）、大豆凝集素（SBA）、槐凝集素（SJA）和荆豆凝集素（UEA）凝集素未观察到结合迹象。观察到三种主要的结合模式：反应产物出现在质膜上（膜状）、局限于细胞膜的一极（帽状）或弥漫性地存在于细胞质中（细胞质状）。呈现膜状和帽状染色模式的细胞对应于淋巴细胞，细胞质状染色的细胞对应于浆细胞和巨噬细胞-组织细胞。在B和T细胞区域的淋巴细胞上，所有六种凝集素均观察到帽状染色。因此，帽状染色的存在可能无助于区分B细胞和T细胞。相比之下，膜状染色仅限于滤泡淋巴细胞（B细胞）与PSA和LCA结合的情况，而生发中心细胞与PHA、WGA、Con A结合，RCA也与T细胞膜发生反应。巨噬细胞-组织细胞的细胞质染色反应因凝集素不同而有显著差异。我们的研究表明，细胞的碳水化合物部分通过常规处理保留了其与凝集素的结合位点，为有效的回顾性研究提供了一种方法。此外，这些观察结果表明，尽管每种凝集素具有相同的抑制糖，但仍应测试其独特的反应模式，这无法从细胞悬液研究的数据中预测。
