Ley R D, Peak M J, Lyon L L
J Invest Dermatol. 1983 Mar;80(3):188-91. doi: 10.1111/1523-1747.ep12534010.
An action spectrum for the induction of pyrimidine dimers in the epidermis of hairless mice was determined between 288 and 307 nm. The presence of pyrimidine dimers in tritium-labeled DNA extracted from exposed SKH:hairless-1 mouse skin was determined using dimer-specific nucleases from Micrococcus luteus in conjunction with sedimentation of the irradiated DNA in alkaline sucrose gradients. The rate of induction of pyrimidine dimers was maximal at 293 nm. These values were used to propose a UVB transmission curve for mouse epidermis.
测定了无毛小鼠表皮中嘧啶二聚体诱导的作用光谱，范围在288至307纳米之间。使用来自藤黄微球菌的二聚体特异性核酸酶，结合经照射的DNA在碱性蔗糖梯度中的沉降，测定了从暴露的SKH：无毛-1小鼠皮肤中提取的氚标记DNA中嘧啶二聚体的存在情况。嘧啶二聚体的诱导率在293纳米处最高。这些值被用于提出小鼠表皮的UVB透射曲线。
