Difficulties in applying the Scatchard model of ligand binding to proteins--proposal of new mathematical tools--application to salicylates.

Ill-considered use of the Scatchard model often leads to unjustified deductions. Since the main difficulty of this model is its number of parameters, new models are proposed that have only two parameters. After checking the models on simulated data, they were applied to real data on the binding of salicylates to albumin.