Wiebe M E, Scherer W F, Peick W J
Am J Epidemiol. 1983 Feb;117(2):201-12. doi: 10.1093/oxfordjournals.aje.a113531.
Ninety-four strains of Venezuelan encephalitis (VE) virus isolated from sentinel hamsters exposed in the Middle American countries of Mexico, Guatemala, Belize, and Honduras were examined for the presence of virions with marker characteristics of strains that cause large epidemics and equine epizootics. Thirty-four strains came from before and 60 strains came from after the Middle American epidemics and equine epizootics of 1966 and 1969-1972. Twenty-three virion clones that resembled epizootic strains by hydroxylapatite chromatography and Vero monkey kidney cell plaque size determinations were characterized further. However, the predominant virions in these clones were like enzootic strains from Middle America north of the Panama Canal region, and not like Middle American epizootic VE strains, since they were in hemagglutination-inhibition antigenic subtype IE, usually had optimal pH of hemagglutination at 6.2, and were avirulent for English shorthair guinea pigs inoculated subcutaneously. These results provide evidence against the theory of origin of epidemic-equine epizootic VE virus strains that posits that epizootic virions emerge in Middle America from strains containing mixtures of enzootic and epizootic virions in enzootic habitats.
对从墨西哥、危地马拉、伯利兹和洪都拉斯等中美洲国家暴露的哨兵仓鼠中分离出的94株委内瑞拉脑炎（VE）病毒进行了检测，以确定是否存在具有导致大规模流行和马 epizootics 的毒株特征性标记的病毒粒子。34株来自1966年以及1969 - 1972年中美洲流行和马 epizootics 之前，60株来自之后。通过羟基磷灰石色谱法和非洲绿猴肾细胞空斑大小测定，对23个类似于 epizootic 毒株的病毒粒子克隆进行了进一步表征。然而，这些克隆中的主要病毒粒子类似于巴拿马运河地区以北中美洲的地方流行毒株，而不像中美洲 epizootic VE 毒株，因为它们属于血凝抑制抗原亚型IE，血凝的最佳pH值通常为6.2，并且对皮下接种的英国短毛豚鼠无毒力。这些结果为反对流行 - 马 epizootic VE 病毒毒株起源理论提供了证据，该理论认为 epizootic 病毒粒子从中美洲地方流行栖息地中含有地方流行和 epizootic 病毒粒子混合物的毒株中出现。
