Roehrig J T, Bolin R A, Hunt A R, Woodward T M
Division of Vector-Borne Infectious Diseases, Centers for Disease Control, Fort Collins, Colorado 80522-2087.
J Clin Microbiol. 1991 Mar;29(3):630-1. doi: 10.1128/jcm.29.3.630-631.1991.
We have prepared a murine monoclonal antibody (MAb) capable of distinguishing between wild-type Venezuelan equine encephalomyelitis (VEE) virus and the TC-83 vaccine derivative. This MAb, 1A2B-10, was derived from immunization with a synthetic peptide corresponding to the first 19 amino acids of the E2 glycoprotein of Trinidad donkey VEE virus. The MAb reacts with prototype viruses from all naturally occurring VEE subtypes except subtype 6 in an enzyme-linked immunosorbent assay. It does not react with TC-83 virus or members of the western and eastern equine encephalitis virus complex or with Semliki Forest virus. This antibody will also differentiate between TC-83 and Trinidad donkey VEE virus in indirect immunofluorescence assays with virus-infected Vero cells.
我们制备了一种鼠单克隆抗体(MAb),它能够区分野生型委内瑞拉马脑炎(VEE)病毒和TC-83疫苗衍生物。这种单克隆抗体1A2B-10是通过用与特立尼达驴VEE病毒E2糖蛋白的前19个氨基酸对应的合成肽免疫制备的。在酶联免疫吸附测定中,该单克隆抗体与除6型以外的所有天然存在的VEE亚型的原型病毒发生反应。它不与TC-83病毒、西部和东部马脑炎病毒复合体的成员或塞姆利基森林病毒发生反应。在对病毒感染的Vero细胞进行的间接免疫荧光测定中,这种抗体也能区分TC-83和特立尼达驴VEE病毒。