Freedman M H, Saunders E F, Cattran D C, Rabin E Z
Am J Kidney Dis. 1983 Mar;2(5):530-3. doi: 10.1016/s0272-6386(83)80095-x.
The anemia of chronic renal failure was studied by assessing the effect of uremic serum on proliferation of human marrow erythroid stem cells into colonies in vitro. Of 50 sera tested, 46 inhibited "CFU-E" colony formation by a mean of 72%, and 42 inhibited "BFU-E" colonies by a mean of 53.5%, compared to normal sera. Analysis of the uremic sera revealed a striking increase of ribonuclease activity in every patient. Mean activity in the study group was 17,346 U/ml serum (range 6,700-36,250) compared to control mean of 1,047 +/- 247 U/ml. Purified ribonuclease added to marrow cultures in concentrations simulating uremic serum produced a dose-dependent decrease in CFU-E colonies suggesting that the substance has a role in the production of anemia of renal failure.
通过评估尿毒症血清对人骨髓红系干细胞在体外形成集落增殖的影响，对慢性肾衰竭贫血进行了研究。在检测的50份血清中，与正常血清相比，46份血清抑制“CFU-E”集落形成，平均抑制率为72%，42份血清抑制“BFU-E”集落，平均抑制率为53.5%。对尿毒症血清的分析显示，每位患者的核糖核酸酶活性显著增加。研究组的平均活性为17346 U/ml血清（范围为6700 - 36250），而对照组的平均活性为1047±247 U/ml。以模拟尿毒症血清的浓度将纯化的核糖核酸酶添加到骨髓培养物中，导致CFU-E集落呈剂量依赖性减少，这表明该物质在肾衰竭贫血的发生中起作用。
