Ekenvall L, Dölling B, Göthe C J, Ebbinghaus L, von Stedingk L V, Wasserman J
Br J Ind Med. 1983 May;40(2):212-5. doi: 10.1136/oem.40.2.212.
Single cell protein (SCP) intended for animal feed purposes was produced in a pilot plant. The SCP consisted of Methylomonas methanolica, a pseudomonas species which is an obligate methanol user. The SCP was cultured in fermenters and later dewatered and dried in a spray-drier. Seven of eight research workers had febrile reactions 6-12 hours after exposure to SCP dust. All workers had high titres of IgG and IgM antibodies against the pseudomonas species as measured with indirect ELISA and passive haemagglutination techniques. The mechanism behind the febrile reaction is judged to be a non-immunological reaction caused by endotoxins. By increasing the particle size of the SCP through using different drying procedures, a product which generated less dust was obtained.
用于动物饲料的单细胞蛋白（SCP）在中试工厂生产。该SCP由甲醇甲基单胞菌组成，它是一种专性利用甲醇的假单胞菌属物种。SCP在发酵罐中培养，随后在喷雾干燥器中脱水和干燥。八名研究人员中有七人在接触SCP粉尘6 - 12小时后出现发热反应。使用间接酶联免疫吸附测定法（ELISA）和被动血凝技术检测发现，所有工人针对该假单胞菌属物种都有高滴度的IgG和IgM抗体。发热反应背后的机制被判定为由内毒素引起的非免疫反应。通过使用不同的干燥程序增加SCP的颗粒大小，获得了产生较少粉尘的产品。
