出血性黄疸钩端螺旋体对人单核白细胞促凝血活性的体外作用：有毒株与无毒株的比较
In vitro effect of Leptospira icterohaemorrhagiae on human mononuclear leukocytes procoagulant activity: comparison of virulent with nonvirulent strain.
作者信息
Miragliotta G, Fumarola D
出版信息
Can J Comp Med. 1983 Jan;47(1):70-2.
The in vitro effect of a virulent and a nonvirulent strain of Leptospira interrogans serotype icterohaemorrhagiae on human peripheral mononuclear cells was investigated. After addition of bacteria to citrated whole blood the production of mononuclear cell procoagulant activity (tissue factor) was observed. Indeed mononuclear cells isolated from whole blood bacteria mixtures after prolonged incubation shortened the recalcification time of normal plasma. The virulent strain induced a significantly higher procoagulant activity than nonvirulent and this effect was dependent on the number of bacteria. The production of tissue factor, a potent trigger of blood coagulation, by Leptospira icterohaemorrhagiae could help to understand the mechanism(s) responsible for the activation of intravascular coagulation associated with leptospirosis.
研究了问号状钩端螺旋体黄疸出血型强毒株和无毒株对人外周血单个核细胞的体外作用。将细菌加入枸橼酸化全血后，观察单核细胞促凝活性（组织因子）的产生。确实，长时间孵育后从全血细菌混合物中分离出的单核细胞缩短了正常血浆的复钙时间。强毒株诱导的促凝活性明显高于无毒株，且这种作用取决于细菌数量。黄疸出血型钩端螺旋体产生组织因子（一种强效的凝血触发因子），这有助于理解与钩端螺旋体病相关的血管内凝血激活机制。
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