Henle K J, Peck J W, Higashikubo R
Cancer Res. 1983 Apr;43(4):1624-7.
The polyols erythritol and adonitol reduced 45 degrees heat killing in asynchronous Chinese hamster ovary cells. Heat protection by glycerol and erythritol increased with the apparent intracellular concentration, as inferred from cell volume measurements, and the number of hydroxyl groups per alcohol molecule. The nonlinear tetrahydroxy alcohol pentaerythritol did not protect but sensitized to heat killing. On cell survival curves, the reduced cell killing of protected cells was expressed by an increased Do for the pentahydroxy alcohol adonitol (0.3 M), whereas equimolar concentrations of glycerol increased primarily the Dq (quasithreshold dose) with little increase in Do. The distribution of Chinese hamster ovary cells within the cell cycle was unaffected by the presence of 0.3 M glycerol in the culture medium. However, the polyols erythritol and sorbitol caused a small but significant loss of cells from the heat-resistant G1 compartment. The cell cycle redistribution with prolonged incubation (6 hr) in polyol-supplemented medium is expected to increase the heat sensitivity of the perturbed cell population; the observed heat protection by polyols suggests that heat resistance in the presence of polyols is not an artifact of an asynchronous cell system. Instead, the data identify a family of heat-protective compounds that may occur naturally in mammalian cells.
多元醇赤藓糖醇和阿东糖醇可降低异步培养的中国仓鼠卵巢细胞的45℃热杀伤作用。根据细胞体积测量结果推断，甘油和赤藓糖醇的热保护作用随表观细胞内浓度以及每个醇分子的羟基数量增加而增强。非线性四羟基醇季戊四醇没有起到保护作用，反而使细胞对热杀伤更加敏感。在细胞存活曲线上，受保护细胞的细胞杀伤减少表现为五羟基醇阿东糖醇（0.3M）的Do增加，而等摩尔浓度的甘油主要增加了Dq（准阈值剂量），Do增加很少。培养基中存在0.3M甘油不会影响中国仓鼠卵巢细胞在细胞周期内的分布。然而，多元醇赤藓糖醇和山梨醇导致耐热的G1期细胞少量但显著减少。预计在添加多元醇的培养基中长时间孵育（6小时）导致的细胞周期重新分布会增加受干扰细胞群体的热敏感性；观察到的多元醇热保护作用表明，多元醇存在时的耐热性并非异步细胞系统的假象。相反，这些数据确定了一类可能天然存在于哺乳动物细胞中的热保护化合物。
