Ryter A, Dedet J P, Rabinovitch M
Exp Parasitol. 1983 Apr;55(2):233-42. doi: 10.1016/0014-4894(83)90017-6.
Bone marrow-derived mouse macrophage cultures infected with Leishmania mexicana amazonensis amastigotes were given a 2-hr pulse with 10 microM phenazine methosulfate (PMS), a cationic electron carrier which destroys the intracellular parasites. Cultures were fixed at different times after the PMS pulse and processed for the detection of acid phosphatase (AcP) activity at the electron microscopic level. Only a small proportion of nontreated, infected macrophages stained for AcP. In contrast, 2 to 6 hr after exposure to PMS, many infected cells displayed AcP-positive lysosomes and parasitophorous vacuoles. This increased AcP reactivity paralleled the reduction in the percentage of morphologically intact parasites. In addition, qualitative observations indicated that while nontreated infected cells contained only few recognizable lysosomes, the lysosomal complement noticeably increased a few hours after exposure to PMS. Most intact intracellular amastigotes were not stained, but damaged parasites were often positive for AcP. Twenty hours after the PMS pulse, the percentage of AcP-positive macrophages dropped to the levels initially present in noninfected cultures and all of the parasites were destroyed. Exposure of noninfected macrophages to PMS did not affect their AcP reactivity.
用亚马逊利什曼原虫无鞭毛体感染的源自骨髓的小鼠巨噬细胞培养物，用10微摩尔吩嗪硫酸甲酯（PMS）进行2小时脉冲处理，PMS是一种可破坏细胞内寄生虫的阳离子电子载体。在PMS脉冲后的不同时间固定培养物，并在电子显微镜水平上进行酸性磷酸酶（AcP）活性检测。未处理的受感染巨噬细胞中只有一小部分对AcP染色。相比之下，在暴露于PMS后2至6小时，许多受感染细胞显示出AcP阳性溶酶体和寄生泡。这种AcP反应性的增加与形态完整的寄生虫百分比的降低平行。此外，定性观察表明，未处理的受感染细胞仅含有少量可识别的溶酶体，而在暴露于PMS后数小时，溶酶体数量明显增加。大多数完整的细胞内无鞭毛体未被染色，但受损的寄生虫通常对AcP呈阳性。PMS脉冲后20小时，AcP阳性巨噬细胞的百分比降至未感染培养物中最初存在的水平，并且所有寄生虫均被破坏。未感染的巨噬细胞暴露于PMS不影响其AcP反应性。
