Mak J W, Lim P K, Sim B K, Liew L M
Exp Parasitol. 1983 Apr;55(2):243-8. doi: 10.1016/0014-4894(83)90018-8.
Cultivation of fourth stage Brugia pahangi and B. malayi larvae from infective larvae (stage 3) were obtained in culture medium RPMI 1640 supplemented with 10% human AB serum and an LCC-MK2 rhesus monkey kidney continuous cell line feeder layer. This culture system kept larvae alive in excess of 7 weeks, and served as a source for collection of the worms' secretory, excretory, and moulting antigens.
在补充有10%人AB血清和LCC-MK2恒河猴肾连续细胞系饲养层的RPMI 1640培养基中，从感染性幼虫（第3阶段）培养出了第4阶段的彭亨布鲁线虫和马来布鲁线虫幼虫。该培养系统使幼虫存活超过7周，并作为收集线虫分泌、排泄和蜕皮抗原的来源。
