Fujimaki Y, Shimada M, Kimura E, Aoki Y
Department of Parasitology, Nagasaki University, Japan.
Parasitol Res. 1988;74(3):299-300. doi: 10.1007/BF00539583.
The effect of diethylcarbamazine (DEC) on infective larvae and immature worms of Brugia pahangi was studied in vitro. The in vitro culture of larvae was done using the technique of Mak et al. (1983). In control cultures, most larvae remained alive for 14 days; over 50% survived until day 22 of cultivation. The addition of DEC did not affect the life span of the larvae. Among those which survived for 22 days in control cultures, 77.8% reached the fourth stage, their length being 2908.2 +/- 453.2 microns. When DEC was added to a final concentration of 0.1 mg/ml, the percentage of larvae attaining the fourth stage was reduced (42.9%) and their growth retarded; the length of the fourth-stage larvae was 2548.4 +/- 414.0 microns. The addition of 1.0 mg/ml DEC completely arrested the growth and development of the larvae.
在体外研究了乙胺嗪（DEC）对彭亨丝虫感染性幼虫和未成熟虫体的影响。幼虫的体外培养采用Mak等人（1983年）的技术。在对照培养物中，大多数幼虫存活14天；超过50%存活至培养的第22天。添加DEC不影响幼虫的寿命。在对照培养物中存活22天的幼虫中，77.8%发育到第四阶段，其长度为2908.2±453.2微米。当添加DEC至终浓度为0.1毫克/毫升时，发育到第四阶段的幼虫百分比降低（42.9%），且其生长受到抑制；第四阶段幼虫的长度为2548.4±414.0微米。添加1.0毫克/毫升的DEC完全抑制了幼虫的生长和发育。
