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基质对连续骨髓培养中集落形成单位 - 细胞（CFU - c）生长的影响。

Effect of the substratum on the growth of CFU-c in continuous marrow culture.

作者信息

Tavassoli M

出版信息

Experientia. 1983 Apr 15;39(4):411-2. doi: 10.1007/BF01963153.

DOI:10.1007/BF01963153
PMID:6832324
Abstract

The effect of the substratum on the maintenance of granulocyte-macrophage progenitors (CFU-c) was studied in continuous mouse marrow culture. Glass and decalcified eggshell membrane were found preferable to plastic, providing better adherence of stromal cells. Eggshell membrane consists of a meshwork of branching fibers suitable for the adherence of stromal cells. The glass surface with its high surface pH may provide an electrostatic attraction for the negatively charged surface of stromal cells.

摘要

在连续的小鼠骨髓培养中研究了基质对粒细胞-巨噬细胞祖细胞（CFU-c）维持的影响。发现玻璃和脱钙蛋壳膜比塑料更适合，能使基质细胞更好地黏附。蛋壳膜由适合基质细胞黏附的分支纤维网络组成。具有高表面pH值的玻璃表面可能对基质细胞带负电荷的表面产生静电吸引。

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Experientia. 1983 Apr 15;39(4):411-2. doi: 10.1007/BF01963153.
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本文引用的文献

1
Morphological studies on long-term culture of marrow cells: characterization of the adherent stromal cells and their interactions in maintaining the proliferation of hemopoietic stem cells.骨髓细胞长期培养的形态学研究：贴壁基质细胞的特性及其在维持造血干细胞增殖中的相互作用。
Am J Anat. 1982 Jun;164(2):91-111. doi: 10.1002/aja.1001640202.
2
Radiosensitivity of stromal cells responsible for in vitro maintenance of hemopoietic stem cells in continuous, long-term marrow culture.
Exp Hematol. 1982 May;10(5):435-43.
3
Decalcified egg shell membrane, a supporting substrate for electron microscopic cross-sections of monolayers of epithelial cell line MDCK.脱钙蛋壳膜，一种用于上皮细胞系MDCK单层电子显微镜横截面的支撑底物。
利用蛋壳膜作为周围神经再生的神经引导通道。
Iran J Basic Med Sci. 2013 Aug;16(8):901-5.
In Vitro. 1971 Jan-Feb;6(4):251-2. doi: 10.1007/BF02625936.
4
Studies on hemopoietic microenvironments. Report of a workshop held in La Jolla,, California, August 8-9, 1974.造血微环境研究。1974年8月8 - 9日于加利福尼亚州拉霍亚举办的研讨会报告。
Exp Hematol. 1975 Aug;3(4):213-26.
5
Differentiation and proliferation of hemopoietic cells in culture.培养体系中造血细胞的分化与增殖。
Methods Cell Biol. 1976;14:387-405. doi: 10.1016/s0091-679x(08)60498-7.
6
Conditions controlling the proliferation of haemopoietic stem cells in vitro.
J Cell Physiol. 1977 Jun;91(3):335-44. doi: 10.1002/jcp.1040910303.