Ponder B A, Wilkinson M M
Dev Biol. 1983 Apr;96(2):535-41. doi: 10.1016/0012-1606(83)90191-4.
We screened cryostat sections of tissues from 10 inbred strains of mice with a panel of conjugated lectins, in a search for polymorphisms which could be used as markers in mouse chimeras. In DDK and RIII mice, but not the other strains tested, there are binding sites on the membrane of vascular endothelial cells for Dolichos biflorus agglutinin (DBA), which is specific for terminal nonreducing N-acetyl galactosamine residues. The expression of DBA binding sites on RIII and DDK endothelium is not, however, uniform: there are consistent differences between blood vessels in different organs. These observations provide evidence of an organ-related heterogeneity in vascular endothelium which is open to biochemical analysis, and which raises the possibility that endothelial cells carry a "tissue address."
我们用一组结合凝集素筛选了来自10个近交系小鼠的组织冷冻切片，以寻找可作为小鼠嵌合体标记的多态性。在DDK和RIII小鼠中，而非其他受试品系中，血管内皮细胞膜上存在对双花扁豆凝集素（DBA）的结合位点，该凝集素对末端非还原N-乙酰半乳糖胺残基具有特异性。然而，RIII和DDK内皮细胞上DBA结合位点的表达并不均匀：不同器官的血管之间存在一致的差异。这些观察结果提供了血管内皮细胞存在与器官相关的异质性的证据，这种异质性易于进行生化分析，并且增加了内皮细胞带有“组织地址”的可能性。
