Ohishi I
Infect Immun. 1983 Apr;40(1):336-9. doi: 10.1128/iai.40.1.336-339.1983.
Two components, designated I and II, of botulinum C2 toxin were injected separately into the same animal. The intravenous injection of one component at different time intervals after intravenous injection of the other component, irrespective of the sequence, was lethal to mice. When components I and II were injected intradermally into separate sites, vascular permeability increased only at the site where component II was injected. The sizes of blued areas were smaller with increased distance between the injection sites of components I and II. When one component was injected intravenously and the other intradermally, an increase in vascular permeability was induced at the intradermal site of injection of component II but not at that of component I. These results indicate that the simultaneous injection of components I and II is not always required to elicit the biological activity of C2 toxin. The vascular permeability response induced by separate injections of the two toxin components suggests that the activity of C2 toxin results from component II binding to the tissue around its injection site and component I recognizing the altered tissue.
将肉毒杆菌C2毒素的两个组分（分别命名为I和II）分别注射到同一动物体内。在静脉注射另一个组分后的不同时间间隔静脉注射其中一个组分，无论顺序如何，对小鼠均具有致死性。当将组分I和II皮内注射到不同部位时，仅在注射组分II的部位血管通透性增加。随着组分I和II注射部位之间距离的增加，蓝色区域的大小变小。当一个组分静脉注射而另一个组分皮内注射时，在组分II的皮内注射部位诱导血管通透性增加，但在组分I的注射部位则没有。这些结果表明，并非总是需要同时注射组分I和II来引发C2毒素的生物活性。两种毒素组分单独注射所诱导的血管通透性反应表明，C2毒素的活性是由于组分II与其注射部位周围的组织结合，以及组分I识别改变后的组织所致。
