Pruess D L, Kellett M
J Antibiot (Tokyo). 1983 Mar;36(3):208-12. doi: 10.7164/antibiotics.36.208.
Streptomyces clavuligerus NRRL 3585, a culture which produces a variety of beta-lactam antibiotics in the penicillin, cephalosporin and clavam families, was found to produce a new beta-lactam antibiotic, Ro 22-5417. The compound, which was neither a substrate for nor inhibitor of several beta-lactamases, showed antimicrobial activity in defined minimal medium but little or no inhibitory activity in nutrient-rich medium. The activity was bacteriostatic against Bacillus species ATCC 27860 and was antagonized by D- and L-methionine, L-cystathionine, L-homocystine and O-acetyl-L-homoserine but not by L-homoserine, L-aspartate, L-cysteine or other common amino acids, vitamins and nucleosides. Our results are consistent with Ro 22-5417 acting as an inhibitor in methionine biosynthesis.
棒状链霉菌NRRL 3585能产生青霉素、头孢菌素和棒酸家族中的多种β-内酰胺抗生素，现已发现它能产生一种新的β-内酰胺抗生素Ro 22-5417。该化合物既不是几种β-内酰胺酶的底物，也不是其抑制剂，在限定的基本培养基中表现出抗菌活性，但在营养丰富的培养基中几乎没有抑制活性。该活性对芽孢杆菌属ATCC 27860具有抑菌作用，且受到D-和L-甲硫氨酸、L-胱硫醚、L-高胱氨酸和O-乙酰-L-高丝氨酸的拮抗，但不受L-高丝氨酸、L-天冬氨酸、L-半胱氨酸或其他常见氨基酸、维生素和核苷的拮抗。我们的结果与Ro 22-5417作为甲硫氨酸生物合成抑制剂的作用一致。
