罗 22 - 5417，一种来自棒状链霉菌的新型棒酸类抗生素。II. 发酵、分离与结构

Ro 22-5417, a new clavam antibiotic from Streptomyces clavuligerus. II. Fermentation, isolation and structure.

作者信息

Evans R H, Ax H, Jacoby A, Williams T H, Jenkins E, Scannell J P

出版信息

J Antibiot (Tokyo). 1983 Mar;36(3):213-6. doi: 10.7164/antibiotics.36.213.

DOI:10.7164/antibiotics.36.213

Abstract

The purification of a new antibiotic, Ro 22-5417, was achieved by a variety of preparative column chromatographic methods. The antibiotic was obtained crystalline with an overall recovery of 3% after a 300-fold purification. The structure was determined from proton and 13C NMR spectra to be 3-(7-oxo-1-aza-4-oxabicyclo[3.2.0]hept-3-yl)alanine.

摘要

通过多种制备柱色谱方法实现了一种新型抗生素Ro 22 - 5417的纯化。经过300倍纯化后，该抗生素获得结晶，总回收率为3%。根据质子核磁共振谱和碳-13核磁共振谱确定其结构为3-(7-氧代-1-氮杂-4-氧杂双环[3.2.0]庚-3-基)丙氨酸。