Barnsley E A
J Bacteriol. 1983 Apr;154(1):113-7. doi: 10.1128/jb.154.1.113-117.1983.
The metabolism of phenanthrene by a gram-negative organism able to use this compound as a sole source of carbon and energy has been examined. 1-Hydroxy-2-naphthoic acid was oxidized by oxygen in a reaction catalyzed by a dioxygenase which was activated by ferrous ions. The stoichiometry of the oxidation and the UV spectrum of the product were consistent with the identification of the product as 2'-carboxybenzalpyruvate. This was confirmed by cleaving the product with a partially purified aldolase to yield 2-carboxybenzaldehyde and pyruvate. A number of enzymes for the metabolism of 1-hydroxy-2-naphthoic acid were induced by growth on phthalate or (less well) by growth on protocatechuate. The latter supported only a slow rate of growth, and this and poor induction may have been due to a slow rate of entry into the cell.
对一种革兰氏阴性菌利用菲作为唯一碳源和能源时菲的代谢情况进行了研究。1-羟基-2-萘甲酸在一种由亚铁离子激活的双加氧酶催化的反应中被氧气氧化。氧化反应的化学计量关系以及产物的紫外光谱与产物为2'-羧基苯甲酰丙酮酸的鉴定结果一致。用部分纯化的醛缩酶裂解产物生成2-羧基苯甲醛和丙酮酸，这证实了上述鉴定。在邻苯二甲酸盐上生长或（程度稍低些）在原儿茶酸上生长可诱导多种代谢1-羟基-2-萘甲酸的酶。后者仅支持缓慢的生长速率，这种情况以及诱导效果不佳可能是由于进入细胞的速率较慢所致。
