Dürre P, Andreesen J R
J Bacteriol. 1983 Apr;154(1):192-9. doi: 10.1128/jb.154.1.192-199.1983.
Cell extracts of Clostridium acidiurici, C. cylindrosporum, and C. purinolyticum converted purine, hypoxanthine, 2-hydroxypurine, 6,8-dihydroxypurine, and uric acid into xanthine by the shortest possible route. Adenine was transformed to xanthine only by C. purinolyticum, whereas the other two species formed 6-amino-8-hydroxypurine, which was neither deaminated nor hydroxylated further. 8-Hydroxypurine was formed from purine by all three species. Xanthine dehydrogenase activity was constitutively expressed by C. purinolyticum. Due to the lability of the enzyme activity, comparative studies could not be done with a purified preparation. All enzymes reported to be involved in formiminoglycine metabolism of C. acidiurici and C. cylindrosporum were present in C. purinolyticum. However, glycine was reduced directly to acetate in all three species, as indicated by radiochemical data and by the detection of glycine reductase in cell extracts of C. cylindrosporum and C. purinolyticum. The expression of glycine reductase and the high ratio of glycine fermented to uric acid present points to an energetic advantage for the glycine reductase system, which is expressed when selenium compounds are added to the growth media.
尿酸梭菌、柱状芽孢梭菌和嘌呤分解梭菌的细胞提取物通过尽可能短的途径将嘌呤、次黄嘌呤、2-羟基嘌呤、6,8-二羟基嘌呤和尿酸转化为黄嘌呤。腺嘌呤仅被嘌呤分解梭菌转化为黄嘌呤，而其他两种菌形成6-氨基-8-羟基嘌呤，该物质既不进一步脱氨基也不进一步羟基化。所有这三种菌都能将嘌呤转化为8-羟基嘌呤。嘌呤分解梭菌组成型表达黄嘌呤脱氢酶活性。由于酶活性不稳定，无法用纯化制剂进行比较研究。据报道，尿酸梭菌和柱状芽孢梭菌中参与亚胺甲基甘氨酸代谢的所有酶在嘌呤分解梭菌中均存在。然而，放射性化学数据以及在柱状芽孢梭菌和嘌呤分解梭菌的细胞提取物中检测到甘氨酸还原酶表明，所有这三种菌都能将甘氨酸直接还原为乙酸盐。甘氨酸还原酶的表达以及发酵的甘氨酸与尿酸的高比例表明，当向生长培养基中添加硒化合物时表达的甘氨酸还原酶系统具有能量优势。
