Jones R, Riding P R, Parker M G
J Endocrinol. 1983 Mar;96(3):407-16. doi: 10.1677/joe.0.0960407.
The relative importance of testosterone and prolactin in regulating growth and protein synthesis in rat accessory sex glands has been investigated. Protein synthesis was measured by incubating tissue minces in vitro with [35S]methionine and analysing labelled proteins on polyacrylamide gels containing sodium dodecyl sulphate. Plasma prolactin was assayed by radioimmunoassay. Results showed that castration for 8 days significantly reduced wet weights and total protein synthesis in the ventral prostate, dorsolateral prostate and caput epididymidis, but that these effects could be reversed by exogenous testosterone. Similarly, the specific incorporation of [35S]methionine into four polypeptides in the ventral prostate, two polypeptides in the dorsolateral prostate and two polypeptides in the caput epididymidis was lowered by castration but markedly stimulated by testosterone. Acute or chronic administration of 2-bromo-alpha-ergocryptine to animals in combination with testosterone had no significant effect on any of the parameters measured, although the drug reduced circulating prolactin to undetectable levels. In addition, exogenous prolactin given alone, or in combination with testosterone, to hypophysectomized rats had no effect on general or specific protein synthesis. The induction of hyperprolactinaemia in immature or mature rats with pituitary homographs had no effect on testosterone-stimulated growth of any accessory gland, although it caused a significant stimulation of total protein synthesis in the dorsolateral prostate and coagulating glands. However, this was a generalized effect as it did not increase the specific incorporation of [35S]methionine into androgen-dependent proteins. The results do not indicate a major role for prolactin in regulating androgen responsiveness of male accessory sex glands in the rat.
已对睾酮和催乳素在调节大鼠附属性腺生长和蛋白质合成中的相对重要性进行了研究。通过将组织碎块与[35S]蛋氨酸在体外孵育，并在含有十二烷基硫酸钠的聚丙烯酰胺凝胶上分析标记蛋白质来测量蛋白质合成。通过放射免疫测定法测定血浆催乳素。结果表明，去势8天显著降低了腹侧前列腺、背外侧前列腺和附睾头的湿重和总蛋白质合成，但这些影响可通过外源性睾酮逆转。同样，去势降低了[35S]蛋氨酸在腹侧前列腺中的四种多肽、背外侧前列腺中的两种多肽和附睾头中的两种多肽中的特异性掺入，但睾酮显著刺激了这种掺入。给动物急性或慢性给予2-溴-α-麦角隐亭并结合睾酮，对所测量的任何参数均无显著影响，尽管该药物将循环催乳素降低至无法检测的水平。此外，单独给予垂体切除的大鼠外源性催乳素，或与睾酮联合给予，对一般或特异性蛋白质合成均无影响。用垂体同源物诱导未成熟或成熟大鼠的高催乳素血症，对睾酮刺激的任何附属性腺生长均无影响，尽管它导致背外侧前列腺和凝固腺中的总蛋白质合成显著增加。然而，这是一种普遍效应，因为它并未增加[35S]蛋氨酸在雄激素依赖性蛋白质中的特异性掺入。结果表明催乳素在调节大鼠雄性附属性腺的雄激素反应性中不起主要作用。
