Droller M J, Gomolka D
J Urol. 1983 Mar;129(3):625-9. doi: 10.1016/s0022-5347(17)52266-1.
Splenic lymphocytes from Fischer rats with carcinogen (FANFT)-induced bladder cancer had depressed natural cytotoxicity that could be enhanced in vitro by the addition of mouse leukocyte interferon to the cytotoxicity assay. Such enhancement appeared to reflect an effect directly on lymphocytes rather than a cytotoxic effect on tumor target cells. The possibility that tumor cell prostaglandin production might partially inhibit lymphocyte cytotoxicity and its enhancement prompted separate attempts to enhance cytotoxicity by inhibiting prostaglandin production during cytotoxicity testing. However, addition of indomethacin to the cytotoxicity assays did not enhance cytotoxicity in lymphocytes from either control or tumor-bearing rats and did not add to the enhancement seen with interferon. Addition to the cytotoxicity assay of unstimulated peritoneal monocytes which themselves have been shown to produce prostaglandins, did not effect lymphocyte cytotoxicity. Correspondingly, indomethacin in parallel samples did not alter baseline levels of cytotoxicity seen. Further stimulation of cytotoxicity by addition of interferon to these samples was also not seen. Taken together, in vitro enhancement of depressed lymphocyte cytotoxicity in tumor-bearing animals was possible with exogenous leukocyte interferon, could not be accomplished by inhibition of prostaglandin production in this system, and did not appear to be influenced by the addition or deletion of monocytes during cytotoxicity testing.
用致癌物（FANFT）诱导膀胱癌的Fischer大鼠的脾淋巴细胞自然细胞毒性降低，在细胞毒性试验中加入小鼠白细胞干扰素可在体外增强这种毒性。这种增强似乎反映了对淋巴细胞的直接作用，而不是对肿瘤靶细胞的细胞毒性作用。肿瘤细胞前列腺素产生可能部分抑制淋巴细胞细胞毒性及其增强作用，这促使在细胞毒性测试期间通过抑制前列腺素产生来单独尝试增强细胞毒性。然而，在细胞毒性试验中加入吲哚美辛并没有增强来自对照或荷瘤大鼠淋巴细胞的细胞毒性，也没有增加干扰素所带来的增强作用。将未刺激的腹腔单核细胞加入细胞毒性试验中，这些单核细胞本身已被证明能产生前列腺素，但这对淋巴细胞细胞毒性没有影响。相应地，平行样本中的吲哚美辛也没有改变所观察到的细胞毒性基线水平。向这些样本中加入干扰素进一步刺激细胞毒性的情况也未出现。综上所述，外源性白细胞干扰素可在体外增强荷瘤动物中降低的淋巴细胞细胞毒性，在该系统中通过抑制前列腺素产生无法实现这一点，并且在细胞毒性测试期间，单核细胞的加入或去除似乎并未对其产生影响。
