Mortimer P P, Humphries R K, Moore J G, Purcell R H, Young N S
Nature. 1983;302(5907):426-9. doi: 10.1038/302426a0.
Viruses have been shown to cause bone marrow aplasia in animals and have been implicated in bone marrow failure in man; however, until recently, a specific link between human viral infection and bone marrow failure has not been proven. In 1975 Cossart and colleagues found a serum parvovirus-like virus (SPLV, sometimes referred to as B19) in human serum. Antibody to this virus is present in the sera of 30-45% of healthy adults (Y. E. Cossart, P. P. Mortimer, unpublished observations). However, evidence for a direct link came from work by Pattison et al. who found five children with transient aplastic crisis of sickle cell disease and evidence of active infection with SPLV. This association was later confirmed in a large series of children with sickle cell disease and aplastic crisis in Jamaica. We have studied the effects of virus-containing material on haematopoiesis, using in vitro colony-forming assays to look for direct evidence for a role of SPLV in bone marrow aplasia. We show here that SPLV-containing sera inhibit erythropoiesis in culture. Moreover, in a child with hereditary spherocytosis who developed transient aplastic crisis, a strong inhibitory effect of the patient's serum on erythropoiesis correlated with the presence of virus.
病毒已被证明可在动物中引起骨髓再生障碍，并被认为与人类的骨髓衰竭有关；然而，直到最近，人类病毒感染与骨髓衰竭之间的具体联系尚未得到证实。1975年，科萨特及其同事在人血清中发现了一种血清细小病毒样病毒（SPLV，有时称为B19）。30%至45%的健康成年人血清中存在针对这种病毒的抗体（Y.E.科萨特、P.P.莫蒂默，未发表的观察结果）。然而，帕蒂森等人的研究提供了直接联系的证据，他们发现五名镰状细胞病患儿出现短暂再生障碍危象，并有SPLV活跃感染的证据。这种关联后来在牙买加的一大系列镰状细胞病和再生障碍危象患儿中得到证实。我们使用体外集落形成试验研究了含病毒物质对造血的影响，以寻找SPLV在骨髓再生障碍中作用的直接证据。我们在此表明，含SPLV的血清在培养中抑制红细胞生成。此外，在一名患有遗传性球形红细胞增多症并出现短暂再生障碍危象的儿童中，患者血清对红细胞生成的强烈抑制作用与病毒的存在相关。
