Jones B, Simpson D P
Ren Physiol. 1983;6(1):19-27. doi: 10.1159/000172877.
The distribution of [14C]-5,5-dimethyl-2,4-oxazolidinedione ([14C]-DMO) was measured in intact rat renal cortex using 22Na+ to estimate extracellular fluid volume. Results of two different methods to obtain tissue were compared, a cortical slice technique and a whole kidney frozen technique. In normal animals with a mean blood pH of 7.41 +/- 0.006 (SEM) the cortical slice method measured an intracellular pH of 6.97 +/- 0.03 (SEM). Corrections are described which can be used to compensate for varying volumes of tubular fluid in the slice and for differing concentrations of intracellular sodium. Using reasonable estimates for tubular fluid volume and intracellular sodium these corrections increase the value of intracellular pH to about 7.20. The results of the study indicate that the cortical slice [14C]-DMO method provides a satisfactory technique to obtain baseline values for intracellular pH in intact renal cortex, which can be used to detect changes in intracellular pH produced by experimental manipulations.
使用22Na+估计细胞外液体积，在完整大鼠肾皮质中测量了[14C]-5,5-二甲基-2,4-恶唑烷二酮（[14C]-DMO）的分布。比较了两种获取组织的不同方法的结果，即皮质切片技术和全肾冷冻技术。在平均血液pH值为7.41±0.006（SEM）的正常动物中，皮质切片法测得细胞内pH值为6.97±0.03（SEM）。描述了一些校正方法，可用于补偿切片中不同体积的肾小管液以及细胞内钠浓度的差异。使用对肾小管液体积和细胞内钠的合理估计，这些校正将细胞内pH值提高到约7.20。研究结果表明，皮质切片[14C]-DMO方法提供了一种令人满意的技术，可用于获取完整肾皮质细胞内pH值的基线值，该值可用于检测实验操作引起的细胞内pH值变化。
