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利用同工酶电泳对肯尼亚骆驼体内伊氏锥虫(锥虫亚属)进行特征分析。

Characterisation of Trypanosoma (Trypanozoon) evansi from camels in Kenya using isoenzyme electrophoresis.

作者信息

Gibson W C, Wilson A J, Moloo S K

出版信息

Res Vet Sci. 1983 Jan;34(1):114-8.

PMID:6836172
Abstract

Forty-nine Trypanozoon stocks isolated from camels in five different areas of Kenya were screened for isoenzyme patterns of 11 enzymes using thin-layer starch gel electrophoresis. The results were compared with those obtained in other studies with Trypanosoma (Trypanozoon) evansi stocks from South America, Nigeria, Sudan and Kuwait. Most of the Kenyan camel stocks were found to be either identical or closely similar to T (T) evansi stocks from elsewhere and were therefore designated T (T) evansi also; these Kenyan stocks grew well in rats and were monomorphic. However, four other Kenyan stocks were electrophoretically distinct, and one of them showed marked pleomorphism in rat blood; this latter stock was shown to be tsetse transmissible and was therefore designated T (T) brucei.

摘要

使用薄层淀粉凝胶电泳对从肯尼亚五个不同地区的骆驼中分离出的49株锥虫属虫株进行了11种酶的同工酶谱筛选。将结果与其他关于来自南美洲、尼日利亚、苏丹和科威特的伊氏锥虫(锥虫属)虫株的研究结果进行了比较。发现大多数肯尼亚骆驼虫株与其他地方的伊氏锥虫虫株相同或非常相似,因此也被指定为伊氏锥虫;这些肯尼亚虫株在大鼠体内生长良好且为单态性。然而,其他四株肯尼亚虫株在电泳上有明显差异,其中一株在大鼠血液中表现出明显的多态性;后一株虫株被证明可通过采采蝇传播,因此被指定为布氏锥虫。

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