一种氰化物解毒剂——二甲基氨基酚对中国仓鼠细胞的致突变性。
The mutagenicity of a cyanide antidote, dimethylaminophenol, in Chinese hamster cells.
作者信息
Lee C G, Webber T D
出版信息
Toxicol Lett. 1983 Apr;16(1-2):85-8. doi: 10.1016/0378-4274(83)90014-0.
The mutagenic activity of a cyanide antidote, dimethylaminophenol (DMAP), has been studied at the hypoxanthine-guanine-phosphoribosyltransferase (HPRT) locus in a line of Chinese hamster cells (V79), using induced resistance to 8-azaguanine as a marker for forward, point mutation. In a replating type of assay DMAP produced a dose-dependent increase in mutation frequency in suspension-treated cells, in the absence of any extrinsic metabolic activation system. The incorporation of a liver microsome preparation raised the concentration of DMAP required to induce mutation, but similar levels of induced mutation frequency (IMF) were produced.
已在中国仓鼠细胞系（V79）的次黄嘌呤 - 鸟嘌呤 - 磷酸核糖转移酶（HPRT）位点研究了氰化物解毒剂二甲氨基苯酚（DMAP）的诱变活性，使用对8 - 氮杂鸟嘌呤的诱导抗性作为正向点突变的标记。在复板类型的试验中，在没有任何外源代谢活化系统的情况下，DMAP使悬浮处理细胞中的突变频率呈剂量依赖性增加。加入肝微粒体制剂提高了诱导突变所需的DMAP浓度，但产生了相似水平的诱导突变频率（IMF）。
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