Srivastava G, Borthwick I A, Brooker J D, May B K, Elliott W H
Biochem Biophys Res Commun. 1983 Jan 14;110(1):23-31. doi: 10.1016/0006-291x(83)91254-8.
Following the recent demonstration [Borthwick, I.A., Srivastava, G., Brooker, J.D., May, B.K. and Elliott, W.H. (1982) Eur. J. Biochem. in press] that chick embryo liver mitochondrial delta-aminolevulinate synthase has a minimum molecular weight of 68,000 (rather than the hitherto accepted value of 49,000), we have shown that the primary translation product of delta-aminolevulinate synthase mRNA is a protein of molecular weight 74,000. This protein has for the first time been shown to occur in the cytosol fraction of drug-treated chick embryo livers. This form does not occur in mitochondria nor does the smaller mitochondrial form occur in the cytosol. It is concluded that the 74,000 molecular weight protein is a precursor which is processed during transport into the mitochondria. In vivo labelling experiments are consistent with this conclusion.
最近的研究表明[博思威克，I.A.，斯里瓦斯塔瓦，G.，布鲁克，J.D.，梅，B.K.和埃利奥特，W.H.（1982年）《欧洲生物化学杂志》即将发表]，鸡胚肝线粒体δ-氨基乙酰丙酸合酶的最小分子量为68,000（而非此前公认的49,000），我们发现δ-氨基乙酰丙酸合酶mRNA的初级翻译产物是一种分子量为74,000的蛋白质。首次证明这种蛋白质存在于经药物处理的鸡胚肝的胞质溶胶部分。这种形式不存在于线粒体中，较小的线粒体形式也不存在于胞质溶胶中。可以得出结论，分子量为74,000的蛋白质是一种前体，在转运到线粒体的过程中会进行加工。体内标记实验与这一结论一致。
