Matsuda T, Watanabe K, Nakamura R
Biochem Biophys Res Commun. 1983 Jan 14;110(1):75-81. doi: 10.1016/0006-291x(83)91262-7.
Ovomucoid and ovoinhibitor were isolated from chicken egg white. Enzyme-linked immunosorbent assay (ELISA) and affinity chromatography of ovoinhibitor-coupled Sepharose 4B showed that about 25% of rabbit anti-ovomucoid antibody reacted with ovoinhibitor. Ovoinhibitor required about 1000 times the concentration of ovomucoid to give 50% inhibition of anti-ovomucoid antibody binding to ovoinhibitor in the plate-binding ELISA. These results suggested that ovoinhibitor possesses antigenic determinants which are conformationally homologous with those of ovomucoid and cross-react with anti-ovomucoid antibodies.
卵类黏蛋白和卵类抑制剂是从鸡蛋白中分离出来的。卵类抑制剂偶联琼脂糖凝胶4B的酶联免疫吸附测定（ELISA）和亲和层析表明，约25%的兔抗卵类黏蛋白抗体与卵类抑制剂发生反应。在平板结合ELISA中，卵类抑制剂需要约卵类黏蛋白浓度1000倍的浓度才能对抗卵类黏蛋白抗体与卵类抑制剂的结合产生50%的抑制。这些结果表明，卵类抑制剂具有与卵类黏蛋白构象同源且能与抗卵类黏蛋白抗体发生交叉反应的抗原决定簇。
