Hu M L, Spallholz J E
Biochem Pharmacol. 1983 Mar 15;32(6):957-61. doi: 10.1016/0006-2952(83)90611-1.
Rat erythrocytes were incubated in vitro with various selenium compounds at 37 degrees. Hemolysis occurred with some selenium compounds but not with corresponding sulfur analogues. Selenite induced more rapid loss of intracellular glutathione (GSH) than did selenocystine but was less hemolytic. Cystine caused neither loss of intracellular GSH nor hemolysis. Addition of GSH to the incubation medium enhanced hemolysis by selenite and selenium dioxide but inhibited hemolysis by selenocystine. Inclusion of glucose in the incubation medium also inhibited selenocystine-induced lysis of erythrocytes from both selenium-supplemented rats and selenium-deficient rats. The results suggest a relationship between the oxidation of intracellular GSH and the hemolysis by selenocystine, selenite and selenium dioxide.
将大鼠红细胞在37℃下与各种硒化合物进行体外孵育。某些硒化合物会导致溶血，而相应的硫类似物则不会。亚硒酸盐比硒代胱氨酸更能迅速导致细胞内谷胱甘肽（GSH）的损失，但溶血作用较小。胱氨酸既不会导致细胞内GSH的损失，也不会引起溶血。向孵育培养基中添加GSH可增强亚硒酸盐和二氧化硒引起的溶血，但会抑制硒代胱氨酸引起的溶血。在孵育培养基中加入葡萄糖也可抑制硒代胱氨酸诱导的补充硒大鼠和缺硒大鼠红细胞的裂解。结果表明细胞内GSH的氧化与硒代胱氨酸、亚硒酸盐和二氧化硒引起的溶血之间存在关联。
