Siwek B, Bahbouth E, Serra M A, Sabbioni E, de Pauw-Gillet M C, Bassleer R
Department of Histology and Cytology, University of Liège, Belgium.
Arch Toxicol. 1994;68(4):246-54. doi: 10.1007/s002040050064.
The effects of selenium compounds such as sodium selenite, sodium selenate, seleno-DL-cystine and seleno-DL-methionine (100 microM and 10 microM) on B16 and pigmented cloned pB16 murine melanoma cells were investigated in vitro. At the tested concentrations, B16 cells showed a greater sensitivity to the toxic effects of sodium selenite and seleno-DL-cystine than pB16 cells, whereas no decrease of B16 and pB16 cell number was observed after incubation with sodium selenate or seleno-DL-methionine. Glutathione (GSH) percentages were strongly decreased only by selenite and seleno-DL-cystine; it was marked more in B16 than in pB16 cells. The pretreatment of B16 cells with a GSH depleting agent (10 microM buthionine-[S,R]-sulfoximine) did not significantly influence the cytotoxic effects of selenite and seleno-DL-cystine. On both cell populations, GSH preincubation (50 microM) enhanced the cytotoxicity of selenite whereas the survival of seleno-DL-cystine treated cells was increased. Glutathione peroxidase (GSH-Px) activity in B16 cells was more sensitive than in pB16 cells to the activating effect of selenite, and particularly of seleno-DL-cystine: however, cell-free controls indicated that activation was mainly due to glutathione reductase. The rate of 75Se (as sodium selenite) uptake in both cell populations was maximal within the first hour of incubation, with a preferential accumulation in the cytosol; after 24 h of incubation, the amount of 75Se in cytosol and pellet was approximately the same.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了亚硒酸钠、硒酸钠、硒代 - DL - 胱氨酸和硒代 - DL - 蛋氨酸（100微摩尔和10微摩尔）等硒化合物对B16和色素沉着的克隆化pB16小鼠黑色素瘤细胞的体外作用。在测试浓度下，B16细胞比亚硒酸钠和硒代 - DL - 胱氨酸的毒性作用对pB16细胞更敏感，而用硒酸钠或硒代 - DL - 蛋氨酸孵育后未观察到B16和pB16细胞数量减少。谷胱甘肽（GSH）百分比仅因亚硒酸盐和硒代 - DL - 胱氨酸而大幅降低；在B16细胞中比在pB16细胞中更明显。用GSH消耗剂（10微摩尔丁硫氨酸 - [S，R] - 亚砜亚胺）预处理B16细胞对亚硒酸盐和硒代 - DL - 胱氨酸的细胞毒性作用没有显著影响。在这两种细胞群体上，GSH预孵育（50微摩尔）增强了亚硒酸盐的细胞毒性，而硒代 - DL - 胱氨酸处理细胞的存活率增加。B16细胞中的谷胱甘肽过氧化物酶（GSH - Px）活性比亚硒酸盐，特别是硒代 - DL - 胱氨酸的激活作用对pB16细胞更敏感：然而，无细胞对照表明激活主要归因于谷胱甘肽还原酶。两种细胞群体中75Se（作为亚硒酸钠）的摄取率在孵育的第一小时内最高，优先积累在细胞质中；孵育24小时后，细胞质和沉淀中75Se的量大致相同。（摘要截断于250字）
