Sillerud L O, Shulman R G
Biochemistry. 1983 Mar 1;22(5):1087-94. doi: 10.1021/bi00274a015.
Natural-abundance 13C NMR signals from glycogen are observable in situ within the perfused livers of rats. The nuclear magnetic relaxation properties (T1, T2, eta + 1) of glycogen were measured for glycogen in situ and in vitro and were found to be identical. All of the carbon nuclei in glycogen contribute to the high-resolution NMR spectrum, in spite of glycogen's very large molecular weight. The metabolism of glycogen in situ in the perfused rat liver was followed by 13C NMR. Stimulation of the fed rat liver by physiological glucagon levels led to rapid glycogenolysis. Perfusion of the liver with [1-13C]glucose led to net glycolysis, with concomitant scrambling of the label from C1 to C6 due to triosephosphate isomerase activity.
在大鼠的灌注肝脏中，可以原位观察到糖原的天然丰度13C NMR信号。对原位和体外的糖原测量了其核磁弛豫特性（T1、T2、eta + 1），发现二者相同。尽管糖原分子量非常大，但其所有碳核都对高分辨率NMR光谱有贡献。通过13C NMR追踪了灌注大鼠肝脏中原位糖原的代谢。生理水平的胰高血糖素刺激喂食大鼠的肝脏会导致快速糖原分解。用[1-13C]葡萄糖灌注肝脏会导致净糖酵解，由于磷酸丙糖异构酶的活性，标记物会从C1快速扩散至C6。
