利用糖原与水的磁耦合进行磁共振成像。
Magnetic resonance imaging of glycogen using its magnetic coupling with water.
机构信息
Russell H. Morgan Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205;
F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD 21205.
出版信息
Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):3144-3149. doi: 10.1073/pnas.1909921117. Epub 2020 Jan 30.
Glycogen plays a central role in glucose homeostasis and is abundant in several types of tissue. We report an MRI method for imaging glycogen noninvasively with enhanced detection sensitivity and high specificity, using the magnetic coupling between glycogen and water protons through the nuclear Overhauser enhancement (NOE). We show in vitro that the glycogen NOE (glycoNOE) signal is correlated linearly with glycogen concentration, while pH and temperature have little effect on its intensity. For validation, we imaged glycoNOE signal changes in mouse liver, both before and after fasting and during glucagon infusion. The glycoNOE signal was reduced by 88 ± 16% ( = 5) after 24 h of fasting and by 76 ± 22% ( = 5) at 1 h after intraperitoneal (i.p.) injection of glucagon, which is known to rapidly deplete hepatic glycogen. The ability to noninvasively image glycogen should allow assessment of diseases in which glucose metabolism or storage is altered, for instance, diabetes, cardiac disease, muscular disorders, cancer, and glycogen storage diseases.
糖原在葡萄糖稳态中起着核心作用，并且在几种类型的组织中大量存在。我们报告了一种使用通过核奥弗豪瑟增强（NOE）的糖原和水质子之间的磁偶联来非侵入性地对糖原进行成像的 MRI 方法，以增强检测灵敏度和特异性。我们在体外表明，糖原 NOE（glycoNOE）信号与糖原浓度呈线性相关，而 pH 值和温度对其强度的影响很小。为了验证，我们在禁食前后以及胰高血糖素输注期间对小鼠肝脏中的 glycoNOE 信号变化进行了成像。禁食 24 小时后，glycoNOE 信号降低了 88 ± 16%（ = 5），腹腔内（i.p.）注射胰高血糖素 1 小时后降低了 76 ± 22%（ = 5），这会导致肝糖原迅速耗尽。非侵入性成像糖原的能力应该可以评估葡萄糖代谢或储存改变的疾病，例如糖尿病、心脏病、肌肉疾病、癌症和糖原贮积病。
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