Kabra P M, Marton L J
Clin Chim Acta. 1983 Feb 28;128(1):9-17. doi: 10.1016/0009-8981(83)90050-5.
We describe a fluorometric liquid chromatographic assay for the measurement of unconjugated estriol in the serum of pregnant women. Estriol is extracted into methylene chloride/propanol-2 from serum by use of a Clin-Elut extraction column, the extract evaporated, and the residue redissolved in mobile phase. An aliquot is injected onto the liquid chromatograph and the estriol is separated on a reversed-phase octyl column with a mobile phase consisting of acetonitrile/phosphate buffer (23:77, v/v). The effluent is monitored by fluorescence detection. The proposed method offers good reproducibility (CV less than 7%), sensitivity (less than 0.5 micrograms/l), and accuracy. Of many drugs and steroids tested, only 16,17-epiestriol interferes with the estriol analysis.
我们描述了一种用于测量孕妇血清中未结合雌三醇的荧光液相色谱法。使用Clin-Elut萃取柱从血清中将雌三醇萃取到二氯甲烷/异丙醇-2中，将提取物蒸发，残渣重新溶解于流动相中。取一份等分试样注入液相色谱仪，在反相辛基柱上用由乙腈/磷酸盐缓冲液（23:77，v/v）组成的流动相分离雌三醇。通过荧光检测监测流出物。所提出的方法具有良好的重现性（CV小于7%）、灵敏度（小于0.5微克/升）和准确性。在测试的许多药物和类固醇中，只有16,17-表雌三醇干扰雌三醇分析。
