Dohji T, Fushimi M, Kawabe T, Kamiyama F, Mori M, Sugita N, Tanizawa O
J Chromatogr. 1984 Nov 28;311(2):249-55. doi: 10.1016/s0378-4347(00)84718-0.
A fluorometric liquid chromatographic method was developed for measurements of unconjugated oestradiol and oestriol in the serum of pregnant women. The serum samples are injected directly into the apparatus and pass to a pretreatment column, where oestrogens are adsorbed while hydrophilic components such as proteins and carbohydrates are not. The oestrogens then pass into a separation column containing a new type of polymer gel. The mobile phase consists of an acetonitrile-water mixture, and separation is achieved by a reversed-phase mechanism. The eluent is monitored for fluorescence. Data on the reproducibility and recovery by this method and the correlation of values with those obtained by radioimmunoassay are reported. Results on the increases of oestradiol and oestriol in the serum during pregnancy are also reported.
建立了一种荧光液相色谱法，用于测定孕妇血清中未结合的雌二醇和雌三醇。血清样本直接注入仪器并进入预处理柱，在预处理柱中雌激素被吸附，而蛋白质和碳水化合物等亲水性成分则不被吸附。然后雌激素进入装有新型聚合物凝胶的分离柱。流动相由乙腈 - 水混合物组成，通过反相机制实现分离。对洗脱液进行荧光监测。报告了该方法的重现性和回收率数据以及与放射免疫分析所得值的相关性。还报告了孕期血清中雌二醇和雌三醇增加的结果。
