Kaplan L A, Hohnadel D C
Clin Chem. 1983 Aug;29(8):1463-6.
Concentrations of unconjugated estriol in maternal serum or plasma increase throughout pregnancy, particularly during the third trimester. We present and discuss the results of a comparative study of unconjugated estriol as measured by a new "high-performance" liquid-chromatographic assay and by a conventional semiautomated radioimmunoassay procedure. In the new method, serum is extracted and chromatographed on a reversed-phase mu Bondapak-C8 column under radial compression. The estriol peak is detected with a glassy-carbon electrochemical detector. The chromatographic results (y) correlated well with the RIA results (x) for 105 samples from 45 pregnant women in their third trimester (y = 1.07x - 0.55 micrograms/L, r = 0.933), with no significant difference between the means of the two methods.
孕期母体血清或血浆中未结合雌三醇的浓度会持续升高，尤其在孕晚期。我们展示并讨论了一项对比研究的结果，该研究采用一种新的“高效”液相色谱分析法和传统的半自动放射免疫分析程序来测定未结合雌三醇。在新方法中，血清经提取后在径向压缩下于反相μ Bondapak-C8柱上进行色谱分析。用玻碳电化学检测器检测雌三醇峰。对45名孕晚期孕妇的105份样本进行分析，色谱分析结果（y）与放射免疫分析结果（x）相关性良好（y = 1.07x - 0.55微克/升，r = 0.933），两种方法的均值无显著差异。
