一种测定肌肉中鹅肌肽和肌肽的新快速方法。

A new rapid method for determination of anserine and carnosine in muscles.

Wołos A, Piekarska K, Pilecka T, Ciereszko A, Jabłonowska C

Comp Biochem Physiol B. 1983;74(3):623-6. doi: 10.1016/0305-0491(83)90239-0.

Abstract

It was found that anserine and carnosine form with o-phthalic aldehyde coloured reaction products of identical molar coefficient of absorption at 640 nm. Hence, a simple and rapid method for spectrophotometric determination of these dipeptides has been worked out. The method was applied to determine the sum of anserine and carnosine in muscles of adult geese, (female). Carnosine was determined with enzymatic method. 2. Ratio between anserine and carnosine concentrations depends on animal age (lower in 1-yr-old geese than in 2- and 3-yr-old ones) and type of muscles (lower in leg muscles compared to breast muscle). 3. Correlation was found between level of carnosine and level of anserine--it depended both on geese and type of muscles.

摘要

发现鹅肌肽和肌肽与邻苯二甲醛形成在640nm处具有相同摩尔吸收系数的有色反应产物。因此，已开发出一种简单快速的分光光度法来测定这些二肽。该方法用于测定成年母鹅肌肉中鹅肌肽和肌肽的总量。肌肽用酶法测定。2. 鹅肌肽和肌肽浓度之间的比例取决于动物年龄（1岁鹅低于2岁和3岁鹅）和肌肉类型（腿部肌肉低于胸肌）。3. 发现肌肽水平和鹅肌肽水平之间存在相关性——这既取决于鹅的种类，也取决于肌肉类型。