Tkachuk V A, Malinovskaia K I
Vopr Med Khim. 1977 Jan-Feb(1):127-31.
A method is described for separation of carnosine and anserine and of the amino acids constituting them by means of thin-layer chromatography. For quantitative estimation the amino acids and dipeptides were treated with 7-chlorine-4-nitrobenzeno-2-hydroxy-1,3-diazol (NBD-chloride) and subsequently separated by chromatography on silica gel and estimated by absorption at 456-470 nm. Systems of solvents were developed for separation of both free and chemically modified amino acids and dipeptides; optimal conditions were chosen for preparation of tissue extracts and their treatment with NBD-chloride. By this method carnosine and anserine could be stimated in extract from 0.02-0.2 mg of muscular tissue. The time required for the analyses was about 3-5 hrs.
本文描述了一种通过薄层色谱法分离肌肽、鹅肌肽及其组成氨基酸的方法。为了进行定量分析，将氨基酸和二肽用7-氯-4-硝基苯-2-羟基-1,3-二唑（NBD-氯化物）处理，随后在硅胶上进行色谱分离，并通过在456 - 470nm处的吸光度进行测定。开发了用于分离游离和化学修饰的氨基酸及二肽的溶剂系统；选择了制备组织提取物及其用NBD-氯化物处理的最佳条件。通过这种方法，可以在0.02 - 0.2mg肌肉组织的提取物中测定肌肽和鹅肌肽。分析所需时间约为3 - 5小时。
