Ishikawa T, Furuno K, Kato K
Exp Cell Res. 1983 Mar;144(1):15-24. doi: 10.1016/0014-4827(83)90436-6.
We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organelles, increased in number in the vicinity of bile canaliculi and also near the Golgi apparatus. At 1 h, most of the autophagic vacuoles were autolysosomes, single membrane-limited bodies positive for acid phosphatase activity. Development of the autolysosomes was accompanied by the reciprocal disappearance of pre-existing secondary lysosomes. From 1 to 8 h, the autolysosomes varied to a great extent in both size and shape as a result of coalescence. Segregated organelles within the autolysosomes were gradually degraded into electron-lucent unidentifiable debris. At later, residual bodies were abundant in the cytoplasm, and occasionally, their contents were discharged into the space of Disse. From 9 to 12 h, the autolysosomes decreased in the volume and number and secondary lysosomes of normal shape and size appeared. The autolysosomes seem to persist for long periods because of a retarded degradation of sequestered materials in leupeptin-treated hepatocytes.
我们采用电子显微镜和细胞化学技术，研究了腹腔注射亮抑酶肽后大鼠肝细胞溶酶体区室的形态学改变。注射后30分钟，含有细胞质细胞器的自噬泡（自噬体和自溶酶体）在胆小管附近以及高尔基体附近数量增加。1小时时，大多数自噬泡为自溶酶体，即对酸性磷酸酶活性呈阳性反应的单膜限界小体。自溶酶体的形成伴随着先前存在的次级溶酶体的相应消失。从1小时到8小时，由于融合，自溶酶体在大小和形状上有很大变化。自溶酶体内隔离的细胞器逐渐降解为电子透明的无法识别的碎片。之后，残余小体在细胞质中大量存在，偶尔其内容物会排入狄氏间隙。从9小时到12小时，自溶酶体的体积和数量减少，出现了正常形状和大小的次级溶酶体。在亮抑酶肽处理的肝细胞中，由于被隔离物质的降解延迟，自溶酶体似乎会长期存在。
