Rønning O W, Lindmo T
Exp Cell Res. 1983 Mar;144(1):171-9. doi: 10.1016/0014-4827(83)90451-2.
We have investigated whether human NHIK 3025 cells are dependent upon a net increase in cellular protein content in order to traverse G1 and S. The increase in DNA and protein content was studied by means of two-parameter flow cytometry using populations of cells synchronized by mitotic selection. By adding 1 microM cycloheximide to the medium protein synthesis was partially inhibited, resulting in negligible net accumulation of protein. The cells were able to enter S and progress through S under such conditions. The latter was the case whether the cells had been accumulating protein during G1 or not. The results further indicate that the larger cells enter S earlier and traverse S at a higher rate than the smaller cells. Our conclusion is that net accumulation of protein does not seem to be a prerequisite for traverse through G1 and S, i.e. DNA replication may be dissociated from the general growth of cell mass.
我们研究了人类NHIK 3025细胞穿越G1期和S期是否依赖于细胞蛋白质含量的净增加。通过使用有丝分裂选择同步化的细胞群体，借助双参数流式细胞术研究了DNA和蛋白质含量的增加情况。向培养基中添加1微摩尔的环己酰亚胺可部分抑制蛋白质合成，导致蛋白质的净积累可忽略不计。在这种条件下，细胞能够进入S期并在S期进展。无论细胞在G1期是否积累了蛋白质，情况都是如此。结果还表明，较大的细胞比较小的细胞更早进入S期并以更高的速率穿越S期。我们的结论是，蛋白质的净积累似乎不是穿越G1期和S期的先决条件，即DNA复制可能与细胞质量的总体增长相分离。
