Sennerstam Roland, Strömberg Jan-Olof
Department of Pathology, Division of Cell Analysis, Karolinska Hospital and Institute, 104 01 Stockholm 60, Sweden.
Institute of Mathematical and Physical Science, University of Tromsø, Tromsø, Norway.
Dev Growth Differ. 1991 Aug;33(4):353-363. doi: 10.1111/j.1440-169X.1991.00353.x.
The relationship between the two subcycles 'the cell-growth cycle' (CGC) and the 'DNA-division cycle' (DDC) were examined in the pluripotent embryonal carcinoma cell line PCC3 N/I. This line shows intraclonal bimodal-like heterogeneity in growth rate. A combined protein (mass) and DNA staining method was used to evaluate the relationship between DDC and CGC at various stages in the cell cycle. The results revealed dissociation of the two subcycles and the mass distributions at certain points in the cell cycle reflected the bimodality reported by us for intermitotic time (IDT) distribution. The results were applied to a model called 'The Two-subcycles Cell Cycle Model' (TSCM). This model predicts that the period of DDC (Pre-S+S-G -M) is fairly constant, while the CGC varies, being the main cause of the growth heterogeneity observed in this line. A point of growth rate regulation (PGRR) in G was thought to coincide with the start of CGC. These results reveal a mechanism by which the nucleo-cytoplasmic ratio of the cells can change from one cell cycle to the next.
在多能胚胎癌细胞系PCC3 N/I中研究了两个子周期——“细胞生长周期”（CGC）和“DNA分裂周期”（DDC）之间的关系。该细胞系在克隆内显示出生长速率的双峰样异质性。采用蛋白质（质量）和DNA联合染色方法来评估细胞周期不同阶段DDC和CGC之间的关系。结果显示这两个子周期相互分离，并且细胞周期中某些点的质量分布反映了我们之前报道的有丝分裂间期时间（IDT）分布的双峰性。这些结果应用于一个名为“双子周期细胞周期模型”（TSCM）的模型。该模型预测DDC的时期（前期-S+S-间隙-M期）相当恒定，而CGC是变化的，这是该细胞系中观察到的生长异质性的主要原因。G期的生长速率调节点（PGRR）被认为与CGC的开始相吻合。这些结果揭示了一种机制，通过该机制细胞的核质比可以从一个细胞周期到下一个细胞周期发生变化。
