Akiyoshi M, Nara T, Hara T
Jpn J Antibiot. 1983 Jan;36(1):182-8.
The present experiment was made to clarify the effect of intravenous administration of micronomicin (sagamicin, MCR) on the inner ear of guinea pigs (300--350 g). MCR was given to the animals intravenously at dose of 25, 50 mg/kg and 100 mg/kg, respectively for 28 days. Pinna reflex test in frequency range from 20 kHz to 0.5 kHz was performed before, during and after the administration. The temporal bones were removed after intravital perfusion of the inner ears with neutral formation after the end of the administration. Histopathological examination was performed on the serial celloidin sections of the inner ears which had been stained with hematoxylin eosin. The following results were obtained. 1. MCR had a mild corti toxicity, which did not show remarkable dose dependent increase. 2. MCR had more affinity for the vestibular organs than for the organ of corti. The affinity for the vestibular organ increased with increase in dose. 3. MCR showed less increase in corti toxicity in intravenous administration than in intramuscular injection. However, the affinity of MCR for the vestibular organ showed little more increase in the intravenous administration than in the intramuscular injection.
本实验旨在阐明静脉注射小诺米星（相模霉素，MCR）对豚鼠（300 - 350克）内耳的影响。分别以25、50毫克/千克和100毫克/千克的剂量给动物静脉注射MCR，持续28天。在给药前、给药期间和给药后进行20千赫至0.5千赫频率范围内的耳廓反射测试。给药结束后，对内耳进行活体灌注中性固定液，然后取出颞骨。对用苏木精伊红染色的内耳连续火棉胶切片进行组织病理学检查。得到以下结果：1. MCR具有轻度的柯替器毒性，且未表现出明显的剂量依赖性增加。2. MCR对前庭器官的亲和力比对柯替器的亲和力更高。对前庭器官的亲和力随剂量增加而增加。3. MCR静脉注射时柯替器毒性的增加比肌肉注射时少。然而，MCR对前庭器官的亲和力在静脉注射时比肌肉注射时略有增加。
