Hummerich W, Konrads A, Roesch R, Sofroniew M
Klin Wochenschr. 1983 Feb 15;61(4):203-8. doi: 10.1007/BF01488976.
A sensitive and specific radioimmunoassay for the measurement of arg-vasopressin (AVP) in human plasma is described. Recovery of added AVP from plasma was about 65-70%. An acetone extraction step was necessary to prevent unspecific blank effects. Sensitivity of the assay is 0.5 pg AVP/ml plasma. In normally hydrated subjects AVP-concentration ranged from 0.7 pg/ml to 5.8 pg/ml and showed a good correlation with plasma osmolality. In patients with complete diabetes insipidus (D.i.) AVP-values were below the sensitivity limit of the method and they were subnormal when D.i. was incomplete. There was no increase of AVP-concentration during fluid restriction in patients with complete or incomplete D.i. In subjects with psychogenic polydipsia AVP-values were normal and dehydration produced adequate rises of plasma AVP. In patients with SIADH (Schwartz-Bartter-syndrome) AVP-values were greatly enhanced (greater than 10 pg/ml) when correlated to plasma osmolality (less than 170 mosmol/kg H2O).
本文描述了一种用于测量人血浆中精氨酸血管加压素（AVP）的灵敏且特异的放射免疫分析方法。血浆中添加的AVP回收率约为65%-70%。需要一个丙酮提取步骤以防止非特异性空白效应。该分析方法的灵敏度为0.5 pg AVP/ml血浆。在正常水合状态的受试者中，AVP浓度范围为0.7 pg/ml至5.8 pg/ml，且与血浆渗透压呈良好的相关性。在完全性尿崩症（D.i.）患者中，AVP值低于该方法的灵敏度极限，在不完全性尿崩症患者中AVP值低于正常水平。在完全性或不完全性尿崩症患者限水期间，AVP浓度没有升高。在精神性多饮症患者中，AVP值正常，脱水时血浆AVP有适当升高。在抗利尿激素分泌不当综合征（施瓦茨-巴特尔综合征）患者中，与血浆渗透压（低于170 mosmol/kg H2O）相关时，AVP值大幅升高（大于10 pg/ml）。
