Chiodera P, Louis F, Legros J J
J Endocrinol Invest. 1984 Aug;7(4):287-93. doi: 10.1007/BF03351004.
A sensitive and specific radioimmunoassay method for simultaneous measurement of plasma arginine vasopressin (AVP) and oxytocin (OT) has been developed utilizing an extraction technique on DEAE Sephadex A25. This procedure resulted in mean recoveries of 70.7% (AVP) and 65.4% (OT) in the peptide range of 5 to 100 pg/4 ml. The sensitivity of the assay is 0.5 pg/tube for AVP and 2 pg/tube for OT. The lower limit of detection for plasma extracts was 1.2 pg AVP/ml and 5 pg OT/ml plasma. Employing this method in normal human non smokers and ad libitum fluid the basal levels (mean +/- SE) of plasma AVP are 3.5 +/- 0.2 pg/ml in males and 4.6 +/- 0.4 pg/ml in females and the basal concentrations of plasma OT are 5.1 +/- 0.3 pg/ml in males and 5.4 +/- 0.3 pg/ml in females. Dehydration and water loading produced significant changes in plasma AVP and OT concentrations and a significant correlation exists between plasma AVP and plasma (r = 0.96, p less than 0.001) and urinary (r = 0.84, p less than 0.01) osmolality, but not between plasma OT concentrations and plasma (r = 0.11, NS) and urinary (r = 0.27, NS) osmolality. These results suggest that a wide range of physiological and pathophysiological changes in plasma AVP and OT can be simultaneously measured by the extraction procedure and the radioimmunoassay described.
利用DEAE葡聚糖A25提取技术，已开发出一种用于同时测量血浆精氨酸加压素（AVP）和催产素（OT）的灵敏且特异的放射免疫分析方法。该方法在5至100 pg/4 ml的肽范围内，AVP的平均回收率为70.7%，OT的平均回收率为65.4%。该分析方法对AVP的灵敏度为0.5 pg/管，对OT为2 pg/管。血浆提取物的检测下限为1.2 pg AVP/ml血浆和5 pg OT/ml血浆。在正常不吸烟且随意饮水的人群中采用该方法，男性血浆AVP的基础水平（均值±标准误）为3.5±0.2 pg/ml，女性为4.6±0.4 pg/ml；男性血浆OT的基础浓度为5.1±0.3 pg/ml，女性为5.4±0.3 pg/ml。脱水和水负荷导致血浆AVP和OT浓度发生显著变化，血浆AVP与血浆渗透压（r = 0.96，p＜0.001）和尿渗透压（r = 0.84，p＜0.01）之间存在显著相关性，但血浆OT浓度与血浆渗透压（r = 0.11，无显著性差异）和尿渗透压（r = 0.27，无显著性差异）之间无相关性。这些结果表明，通过所述的提取程序和放射免疫分析可同时测量血浆AVP和OT的广泛生理和病理生理变化。
