Jaffery N F, Ahmad S N, Jailkhani B L
J Pharmacol Methods. 1983 Feb;9(1):33-9. doi: 10.1016/0160-5402(83)90048-7.
A 0.5 ml sample of serum, containing different antiepileptic drugs, singly or in combination, was made alkaline, overlayered with isooctane, and steamed in the presence of KMnO4. The spectra of oxidized products in the organic layer were recorded in the UV range. Oxidized phenobarbitone and primidone show no absorption peak; diazepam a delta-max at 228 nm; phenytoin at 247 nm; and carbamazepine at 247 nm and 372 nm. Consequently, phenobarbitone and diazepam do not interfere in phenytoin quantitation, but carbamazepine does. The contribution of carbamazepine at 247 nm was calculated from the absorption at 372 nm and the ratio of its molar extinction coefficients at the two wave lengths. This was subtracted from the total A247 values to get the actual values due to phenytoin. Thus, a method for simultaneous analysis of carbamazepine and phenytoin in a single sample has been developed.
取一份0.5毫升含有不同抗癫痫药物（单独或联合使用）的血清样本，使其呈碱性，覆盖异辛烷，并在高锰酸钾存在下进行蒸干。记录有机层中氧化产物在紫外范围内的光谱。氧化后的苯巴比妥和扑米酮无吸收峰；地西泮在228纳米处有最大吸收峰；苯妥英在247纳米处有最大吸收峰；卡马西平在247纳米和372纳米处有最大吸收峰。因此，苯巴比妥和地西泮不干扰苯妥英的定量分析，但卡马西平会干扰。根据372纳米处的吸收以及其在两个波长下的摩尔消光系数之比，计算出卡马西平在247纳米处的贡献。从总A247值中减去该贡献，以得到苯妥英的实际值。因此，已开发出一种同时分析单个样本中卡马西平和苯妥英的方法。
