Laerum F
Radiology. 1983 Jun;147(3):681-4. doi: 10.1148/radiology.147.3.6844604.
Cultured endothelial cells derived from human umbilical cord veins were labeled with Na2 (51Cr)O4 and exposed to pure isoiodinated contrast media or control solutions for 10 minutes to simulate the effects of intravenous injections of contrast media on the human endothelium. Metrizamide, iohexol, iopamidol, meglumine/Ca metrizoate (300 mg I/ml), meglumine/Na diatrizoate, meglumine/Na ioxaglate, and meglumine/Ca metrizoate (200 mg I/ml) were tested. Iso-osmolal mannitol solutions were used to determine the portion of injury that was due to the effects of osmolality. Up to six times more chromium-51 was released from the cultures following exposure to the two hyperosmolal ionic contrast media (meglumine/Na diatrizoate and meglumine/Ca metrizoate) than with exposure to the least toxic agent, iopamidol. The dimer ioxaglate and the three nonionic agents (iohexol, metrizamide, and iopamidol) gave a chromium-51 release rate that was almost equal to or lower than that of 0.9% saline. Follow-up of the washed cultures for 24 hours showed no residual toxic effects.
将源自人脐静脉的培养内皮细胞用Na2(51Cr)O4标记，然后暴露于纯的等碘代造影剂或对照溶液中10分钟，以模拟静脉注射造影剂对人体内皮的影响。测试了甲泛葡胺、碘海醇、碘帕醇、葡甲胺/钙泛影葡胺（300mg碘/毫升）、葡甲胺/泛影酸钠、葡甲胺/碘克沙葡胺钠以及葡甲胺/钙泛影葡胺（200mg碘/毫升）。使用等渗甘露醇溶液来确定由渗透压影响所导致的损伤比例。与暴露于毒性最小的试剂碘帕醇相比，暴露于两种高渗离子型造影剂（葡甲胺/泛影酸钠和葡甲胺/钙泛影葡胺）后，培养物中释放的铬-51最多可高出六倍。二聚体碘克沙葡胺和三种非离子型试剂（碘海醇、甲泛葡胺和碘帕醇）的铬-51释放率几乎等于或低于0.9%生理盐水的释放率。对洗涤后的培养物进行24小时随访，未发现残留毒性作用。
