Laerum F, Börsum T, Reisvaag A
Invest Radiol. 1983 Mar-Apr;18(2):199-206. doi: 10.1097/00004424-198303000-00020.
Endothelial cells in primary cultures from human umbilical cord veins were incubated with various radiographic contrast media in increasing concentrations up to approximately 60 mgI/ml for 24 hours in order to study their toxicity. Cell death was recorded with the chromium-51 (51Cr)-release method and controlled by dye exclusion tests, Coulter counting, and protein determination. The hyperosmolar, ionic contrast medium, meglumine metrizoate, was far more toxic to the endothelium than the nonionic media, metrizamide and iohexol, which are far less hyperosmolar. The 51Cr-release test on endothelial cultures provides a simple and useful technique in the evaluation of various intravascular contrast media and their components.
为研究毒性，将来自人脐静脉的原代培养内皮细胞与浓度不断增加（最高约60 mgI/ml）的各种放射造影剂一起孵育24小时。用铬-51（51Cr）释放法记录细胞死亡情况，并通过染料排除试验、库尔特计数和蛋白质测定进行对照。高渗离子型造影剂葡甲胺泛影葡胺对内皮的毒性远大于非离子型造影剂甲泛葡糖和碘海醇，后两者的渗透压要低得多。对内皮细胞培养物进行的51Cr释放试验为评估各种血管内造影剂及其成分提供了一种简单而有用的技术。
