Levander O A, Alfthan G, Arvilommi H, Gref C G, Huttunen J K, Kataja M, Koivistoinen P, Pikkarainen J
Am J Clin Nutr. 1983 Jun;37(6):887-97. doi: 10.1093/ajcn/37.6.887.
Three groups of 10 men of low selenium status were given 200 micrograms Se/day as Serich wheat, Se-rich yeast, or sodium selenate for 11 wk. Twenty unsupplemented subjects served as controls. Plasma Se levels increased steadily in the wheat and yeast groups for 11 wk without plateauing, whereas in the selenate group, plasma Se plateaued around 110 ng/ml after 4 wk. Platelet glutathione peroxidase (GSH-Px) activities increased rapidly in the wheat and selenate groups for 4 wk and then plateaued. Platelet GSH-Px increased more slowly in the yeast group. Ten weeks after the supplements were discontinued, platelet GSH-Px was higher in the wheat and yeast groups than in the selenate group. Assessment of Se bioavailability requires a short-term platelet GSH-Px measurement to determine immediate availability, a medium-term plasma Se measurement to estimate retention, and a long-term platelet GSH-Px measurement after supplements are discontinued to determine the covertibility of tissue Se stores to biologically active Se.
将三组10名低硒状态的男性，分别给予富含硒的小麦、富硒酵母或硒酸钠，剂量为每天200微克，持续11周。20名未补充硒的受试者作为对照。小麦组和酵母组的血浆硒水平在11周内持续上升，没有达到平稳状态，而在硒酸钠组，4周后血浆硒在110纳克/毫升左右达到平稳。小麦组和硒酸钠组的血小板谷胱甘肽过氧化物酶（GSH-Px）活性在4周内迅速增加，然后趋于平稳。酵母组血小板GSH-Px增加得更慢。停止补充10周后，小麦组和酵母组的血小板GSH-Px高于硒酸钠组。评估硒的生物利用度需要进行短期血小板GSH-Px测量以确定即时可用性，进行中期血浆硒测量以估计留存情况，以及在停止补充后进行长期血小板GSH-Px测量以确定组织硒储备转化为生物活性硒的能力。
