Marsh W L, Nelson D P, Koenig H M
Am J Clin Pathol. 1983 Jun;79(6):655-60. doi: 10.1093/ajcp/79.6.655.
Since the early 1970s, measurement of free erythrocyte protoporphyrin (FEP) (by microextraction or by hematofluorometer) has been widely used to screen for lead poisoning and to evaluate microcytic red blood cell (RBC) disorders in children. However, published information on normal values for FEP, performance of the hematofluorometer, and usefulness of FEP in classifying microcytic RBC disorders in adults is scant. In an adult population with normal hematocrit and MCV and normal values for serum ferritin, blood lead level, and serum bilirubin, we obtained normal hematofluorometer FEP levels of less than 30 micrograms/dL for men, and less than 40 micrograms/dL levels for women. We have evaluated a hematofluorometer against a standard microextraction procedure and feel that the hematofluorometer is superior for clinical laboratories if elevated FEPs are confirmed by retesting with washed RBC. In the second part of this paper we demonstrate the usefulness of the FEP in classifying microcytic RBC disorders in adults.
自20世纪70年代初以来，（通过微量萃取法或血液荧光计）测定游离红细胞原卟啉（FEP）已被广泛用于筛查儿童铅中毒以及评估儿童小细胞性红细胞（RBC）疾病。然而，关于FEP正常值、血液荧光计性能以及FEP在成人小细胞性RBC疾病分类中的作用的公开信息却很少。在一组血细胞比容和平均红细胞体积（MCV）正常、血清铁蛋白、血铅水平和血清胆红素值也正常的成年人群中，我们得出，男性血液荧光计FEP正常水平低于30微克/分升，女性低于40微克/分升。我们已将一台血液荧光计与标准微量萃取法进行了对比评估，并且认为，如果通过用洗涤过的RBC重新检测确认FEP升高，那么血液荧光计对临床实验室来说更具优势。在本文的第二部分，我们展示了FEP在成人小细胞性RBC疾病分类中的作用。
