Cobel-Geard R J, Hassouna H I
Am J Hematol. 1983 May;14(3):227-33. doi: 10.1002/ajh.2830140304.
Protamine sulfate (salmine), a basic protein with a molecular weight of 4,626 +/- 109, is a known antiheparin agent which in the absence of heparin demonstrates an anticoagulant activity. To date, much work has been done to elucidate the interaction of heparin with thrombin and its physiologic inhibitor, Antithrombin III (ATIII). Little is known, however, about the mechanism of anticoagulant action of protamine sulfate and its mode of thrombin inactivation. We provide information about the interaction of protamine sulfate with purified, labeled thrombin and ATIII through binding experiments in which protamine is shown to inhibit the inactivation of thrombin by ATIII. Furthermore, we show in clotting assays that protamine sulfate has an inhibitory effect on thrombin in the conversion of fibrinogen to fibrin, and that this inhibition is concentration dependent, partial, and reversible.
硫酸鱼精蛋白（精蛋白）是一种分子量为4,626±109的碱性蛋白质，是一种已知的抗肝素剂，在没有肝素的情况下具有抗凝活性。迄今为止，已经开展了大量工作来阐明肝素与凝血酶及其生理抑制剂抗凝血酶III（ATIII）之间的相互作用。然而，关于硫酸鱼精蛋白的抗凝作用机制及其使凝血酶失活的方式却知之甚少。我们通过结合实验提供了有关硫酸鱼精蛋白与纯化的、标记的凝血酶和ATIII相互作用的信息，其中显示鱼精蛋白可抑制ATIII使凝血酶失活。此外，我们在凝血试验中表明，硫酸鱼精蛋白在纤维蛋白原转化为纤维蛋白的过程中对凝血酶具有抑制作用，并且这种抑制作用是浓度依赖性的、部分性的且可逆的。
